dat <- data.frame(a = "sdsds",flag = 1)
dat$flag <- sprintf('')
library(knitr)
kable(dat)
| a | flag |
|---|---|
| sdsds |
If you haven’t already, download the Excel template here.
If you don’t have access to Microsoft Excel, you can use any other spreadsheet software such as: Apache OpenOffice™ “Calc”, which can be found at www.openoffice.org. When saving the file, ensure you save it as type “Microsoft Excel 97/2000/XP (.xls or .xlsx)”.
Before applying bacteria: 1. Remove target plate from holder and briefly rinse with HPLC grade methanol. 2. Air-dry, prevent touching or contaminating the top surface of the plate (the side with writing and MALDI-spot circles)
When applying bacteria to MALDI plate, your spot should resemble column 2. Column 1 has a little cell material, and column 3 has too much cell material
| 1. Apply bacteria directly without any prior chemical treatment. Smear a single bacterial colony in a thin layer directly onto the MALDI target plate using a sterile toothpick. | |
| 2. Leave the Calibration spots empty. | |
| 3. Add 1 µL of 70% formic acid to each spot containing bacteria. Let air dry. | |
| 4. Add 1 µL of MALDI matrix to each bacterial spot, let air dry. |
The MALDI plate should be properly cleaned before shipping. In order to clean the MALDI plate, use the steps below: method adapted from Freiwald & Sauer
Each plate will require multiple calibration spots. For protein data, follow the directions in the pdf link below: - Bruker Biotyper calibration procedure: - https://www.bruker.com/fileadmin/user_upload/8-PDF-Docs/Separations_MassSpectrometry/InstructionForUse/IFU_Bruker_Bacterial_Test_Standard_Revision_C.pdf
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Dr. Brian T. Murphy
900 S. Ashland Ave. M/C 870
MBRB 3114
Chicago, IL 60607
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Address:
Contains:
Return Address:
Dr. Brian T. Murphy
900 S. Ashland Ave. M/C 870
MBRB 3114
Chicago, IL 60607